Various lysates were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Cyclin D1 (26939-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin D1 antibody (26939-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Cyclin D1 antibody (26939-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Non-treated HeLa and thymidine treated HeLa cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

WB result of Cyclin D1 antibody (60186-1-Ig, 1:12,000) with si-Control and si-Cyclin D1 transfected HeLa cells.

1X10^6 SH-SY5Y cells were stained with 0.2 ug Anti-Human Cyclin D1 (60186-1-Ig, Clone:2G3G5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Cardinal Red™ Anti-Human Cyclin D1 (CR-60186, Clone:2G3G5) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human Cyclin D1 (CL488-60186, Clone:2G3G5) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Cyclin D1 (CL488-26939) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Cyclin D1 (CL647-60186, Clone:2G3G5) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Cyclin D1 fusion protein Ag23228 SDS-PAGE

Cyclin D1 fusion protein Ag23241 SDS-PAGE